Polimery wykorzystywane do rekonstrukcji kości - ocena wybranych podłoży polimerowych w hodowli in vitro osteoblastów

W. Fabianowski; B. Polak; M. Lewandowska-Szumieł

Published : 2022-09-01

Vol. 49 No. 7-8 (2004)

Polymers for bone reconstruction - evaluation of chosen polimeric substrates in osteoblasts in vitro culture

W. Fabianowski B. Polak M. Lewandowska-Szumieł

Abstract

In a literature review the theoretical fundamentals and achievements in osseous implants' investigations have been presented. Especially polymeric materials used in implants were discussed as well as the criteria of the proper polymers choice. These criteria cover the structure of a polymer, porosity, mechanical and surface properties and biodegradation process course. Modification of osseous implant surface with proteins has been also discussed. The part concerning the own research contains the results of evaluation of bone cells' (osteoblasts) adhesion to various types of biomaterials, used as porous osseous implants. A new way of evaluation, presented in Scheme A, was used. Contact angle values (CA, Fig. 1) of the surfaces obtained were measured. Also viability of cells after one-week incubation was investigated basing on the measurements of activity of enzymes of succinate dehydrogenase (XTT Test, Fig. 2) or alkaline phosphatase (ALP Test, Fig. 3). 11 series of measurements differing in the substrate type were done. The best results have been obtained for the surfaces with dextran as well as with the systems: dextran + poly(acrylic acid) (PAA), dextran + Ca(NO3)2 or dextran + PAA + Ca(NO3)2. We did not find an unambiguous correlation between biological tests results and hydrophylic or hydrophobic character of polymeric substrate.

Keywords

implanty kostne ; metoda oceny przydatności biopolimerów ; osadzanie ; komórka kostna ; test XTT ; test ALP ; hydrofobowość podłoża polimerowego ; hydrofilowość podłoża polimerowego osseous implants ; method of estimation of biopolymers' ; XTT Test ; ALP Test ; hydrophobic character of polymeric substrate ; hydrophylic character of polymeric substrate ; bone cells deposition

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Fabianowski, W., Polak, B., & Lewandowska-Szumieł, M. (2022). Polymers for bone reconstruction - evaluation of chosen polimeric substrates in osteoblasts in vitro culture. Polimery, 49(7-8), 522–529. Retrieved from https://polimery.ichp.vot.pl/index.php/p/article/view/1780

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W. Fabianowski wofab@ch.pw.edu.pl

Affiliation
Politechnika Warszawska, Wydział Chemiczny, ul. Noakowskiego 3, 00-664 Warszawa

B. Polak

Affiliation
Politechnika Warszawska, Wydział Chemiczny, ul. Noakowskiego 3, 00-664 Warszawa

M. Lewandowska-Szumieł

Affiliation
Akademia Medyczna w Warszawie, Wydział Nauk o Zdrowiu, ul. Chałubińskiego 5, 02-004 Warszawa