Akryloamidowe pochodne skrobi w układach dwufazowych służących do oczyszczania białek. Cz. II. Zastosowanie do oczyszczania inhibitora alfa-amylazy z mąki pszennej

Z. Brzozowski; N. Pietruszka; v I. Galae; B. Mattiasson

doi:10.14314/polimery.2000.707

Published : 2022-09-21

Vol. 45 No. 10 (2000)

Acrylamide derivatives of starch in two-phase systems used for purification of proteins. Part II. The use for purification of the wheat flour derived alpha-amylase inhibitor

Z. Brzozowski N. Pietruszka v I. Galae B. Mattiasson

DOI: https://doi.org/10.14314/polimery.2000.707

Abstract

Preparation and properties of vinyl imidazole (VI)/vinyl caprolactam (VCL) copolymers and their complex compounds with copper(II) are described, which are capable of selectively separating some proteins in mixtures. The temperature of precipitation of these copolymers from aqueous solutions (Figs. 1-3) was studied in relation to the pH and NaCl content in the solution. A method was developed to prepare a Cu(II)-VI/VCL 10:90* complex able to form a two-phase system with starch acrylamide derivatives (APS) in the presence of NaCl. The two-phase system containing the Cu(II)-VI/VCL = 10:90* complex and an S/AA = 80:60 APS was found to help isolate a pure a-amylase inhibitor from wheat flour in 75% yield (Table 2). The acrylamide derivatives of starch were considered to be excellent cheap substitutes for expensive dextran and useful for the purification of various substances in aqueous two-phase systems.

Keywords

kopolimery VI/VCL ; kompleksy z Cu(II) ; temperatura wytrącania z roztworów wodnych ; układy dwufazowe ; oczyszczanie inhibitora alfa-amylazy VI/VCL copolymers ; complexes with Cu(II) ; temperature of precipitation from aqueous solutions ; two-phase systems ; purification of a-amylase inhibitor

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Brzozowski, Z., Pietruszka, N., Galae, v I., & Mattiasson, B. (2022). Acrylamide derivatives of starch in two-phase systems used for purification of proteins. Part II. The use for purification of the wheat flour derived alpha-amylase inhibitor. Polimery, 45(10), 707–712. https://doi.org/10.14314/polimery.2000.707

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Z. Brzozowski polimery@ichp.pl

Affiliation
Politechnika Warszawska, wydział chemiczny, Zakład Chemii Fizycznej Polimerów i Tworzyw Specjalnych, ul. Noakowskiego 3, 00-664 Warszawa

N. Pietruszka

v I. Galae

B. Mattiasson